Journal: Cells
Article Title: TGF-β1 Promotes Zika Virus Infection in Immortalized Human First-Trimester Trophoblasts via the Smad Pathway.
doi: 10.3390/cells11193026
Figure Lengend Snippet: Figure 1. Expression of Tyro3 and AXL on the surface Swan.71 cells. (A) Immunofluorescence assay. The cells were cultured on glass coverslips in 6-well plates and stained for Tyro3 or AXL. Scale bar: 100 µM. (B) Flow cytometry analysis. Trophoblast cells were cultured in a 96-well plate and stained for Tyro3 or AXL. Numbers displayed inside each panel correspond to the percentage of positive cells for Tyro3 or AXL of the parent-gated population. (A,B) Data obtained from Vero cells performed in parallel were used as comparative references.
Article Snippet: After blocking, the cells were incubated with one of a primary antibody, a rabbit polyclonal anti-Tyro3 antibody (Proteintech, Rosemont, IL, USA), or a rabbit polyclonal anti-AXL antibody (GTX129407, GeneTex), followed by the previously mentioned second antibody (ab150081, Abcam).
Techniques: Expressing, Cell Culture, Staining, Flow Cytometry